Fisher Taq Polymerase
The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It ensures higher sensitivity longer PCR products and higher yields compared to conventional Taq DNA polymerase.
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The specially optimized Thermo Scientific DreamTaq Buffer enables.
Fisher taq polymerase. Fisher BioReagents Taq DNA Polymerase Allows up to 100 cycles of amplification without loss of activity 12600 - 121500. Taq buffer with KCl and Taq buffer with NH 4 2 SO 4. Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications.
Taq DNA Polymerase is supplied with two buffers. Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase available in standard and hot-start formats that offers a balance between performance and value. Ad Request A Quote Or Sample.
Supplied as 500 U 1 U per µ. Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for standard PCR applications. AS ONE Taq DNA Polymerase with red dye is a thermostable recombinant DNA polymerase which exhibits very high activity in primer extension and other molecular biology applications.
Highlights Thermostablehalf life is more than 40 min at 95C Generates PCR products with 3-dA overhangs Supplied with two buffers10X Taq Buffer with KCl and 10X Taq Buffer with NH 4 2 SO 4. It ensures higher sensitivity longer PCR products and higher yields compared to conventional Taq DNA polymerase. Ideal for standard PCR of amplicons 5 kb or shorter.
Platinum Taq DNA High Fidelity Polymerase 5 UµL 02 µL 1 U 1 U is sufficient for amplifying most targets. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming. If you are viewing this page as a.
Ad A highly thermostable recombinant DNA polymerase that catalyzes 5 to 3 synthesis of DNA. Mix and briefly centrifuge the components. Applications include PCR-based DNA amplification cycle sequencing amplification of cloned inserts in vectors Microarray applications primer extensions and all applications requiring high specificity and hot-start PCR.
3 Add template DNA and primers. Pluthero FG Nucleic Acids Res. Ad Request A Quote Or Sample.
PCR Polymerase At 003 Per Unit. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter. In some cases more enzyme may be required up to 25 units.
PCR Polymerase At 003 Per Unit. Use 1 U of enzyme for targets 12 kb. Taq Purification For the Fisher dialysis we have 35 cm is enough for 20ml allow an additional 2-4 cm on both ends for clamps so totally 6 cm will do.
It helps ensure higher yields and sensitivity Figure 1 and longer PCR products than conventional Taq DNA polymerases. Rapid purification of high-activity Taq DNA polymerase. The enzyme catalyzes 53 synthesis of DNA has no detectable 35 exonuclease proofreading activity and possesses low 53 exonuclease activity.
Ad A highly thermostable recombinant DNA polymerase that catalyzes 5 to 3 synthesis of DNA. Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. DreamTaq DNA polymerases are supplied with specially optimized buffers that enable robust DNA amplification with minimal optimization of reaction conditions.
K stabilizes primer annealing whereas NH 4 has a destabilizing effect especially on weak hydrogen bonds between mismatched primer - template base pairs. Therefore for standard PCR with Taq DNA Polymerase and 02 mM dNTPs the recommended MgCl 2. Taq Ti -Heat-activated DNA Polymerase.
Ideal for standard PCR of amplicons 5 kb or shorter. Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. DreamTaq DNA Polymerase uses the same reaction set-up and cycling conditions as conventional Taq DNA polymerase.
One unit of TaqDNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74C This product s resides on a Fisher Scientific GSA or VA contract. It has a 53 DNA polymerase activity and a 53 exonuclease activityWith our Taq DNA. DreamTaq DNA Polymerase uses the same reaction set-up and cycling conditions as conventional Taq DNA polymerase.
Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. Supplied as 500 U 1 U per µ. Description DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications.
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